(a) Representative density plot comparing side (SSC-A) versus forward scatter area (FSC-A). 562245), the cells were stained according to the kit instructions and acquired on a BD Accuri™ C6 flow cytometer using the kit template. No. Select from the following table: BD Accuri C6 system (32-bit) software is not forward compatible with BD Accuri C6 Plus (64-bit) software and the FCS 3.1 file format. 560249) according to the kit procedure. StatLock™ IV Ultra Stabilization Device secures non-winged B. Braun and BD safety catheters. Many factors, including stress, radiation, environmental exposure, and treatment with small molecules, can lead to changes in cell cycle, apoptosis, DNA damage, and cell proliferation. Using the BD Stemflow™ Human MSC Analysis Kit (Cat. 11. Start Date 18 Dec, 2020 (Yesterday) Due Date 02 Jan, 2021 (in 13 days) Opportunity Type Bid Notification. Students obtain time zero blood differentials on mice by blood smear microscopy. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open) 10. After harvesting and staining the cells with 7-AAD and either FITC or APC anti-BrdU according to the kit protocol, samples were acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. 60 0 obj <>stream Because intracellular analysis requires permeabilization, surface marker analysis is essential when researchers want to isolate live cell populations for further analysis. Just lift the transparents "gull-wings," place catheter wings over the posts, and close. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. On BD Accuri™ flow cytometers, triggers and thresholds help to accurately identify small particles such as microbes. A primary challenge of stem cell research is the inherent heterogeneity of the cell cultures. For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User … An open, non-pressurized fluidics system makes BD Accuri systems ideal for kinetic studies. Figure 3-2. U-937 cells treated with IFN-γ showed increased Stat1 (pY701) phosphorylation in a dose-dependent manner. Accurate cell concentrations are essential in many research applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring microorganism concentrations in purified water, and determining the viability of cultured cell lines. Figure S1. Samples were collected on the BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software. Cellular processes and events are probabilistic and take place in a context of complex varying environments, which they influence and which influence them. A high correlation between PI-DNA emission in the FL2 and FL3 channels places the nuclear events in a narrow diagonal region from which it is easy to exclude fragments and debris. Interference Filter. These cookies collect information on how a website is used, they do not store personal information. Using panels of directly conjugated fluorescent antibodies to recognize specific cell surface and intracellular epitopes, multicolor flow cytometric analysis allows researchers to interrogate specific target protein levels expressed by individual cells in various phases of development and differentiation. For walkaway convenience, the optional BD CSampler Plus accessory offers reliable and easy-to-use automation. This site uses cookies. Compact and rugged, they can be transported into labs and processing facilities, and even into the field. By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. 9. BD Accuri C6 - Information and resources for the predecessor BD Accuri C6 personal flow cytometer, Enhanced sensitivity, reliability and capability, Simplicity: easy to learn, use, maintain and teach, Wide applications in areas such as immunology, cell and cancer biology, plant and microbiology, and education. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. Manual Provides Information On Cytometer Requirements And Specifications, Setup, And Maintenance. Cell survival, growth, and differentiation are tightly regulated through expression and post-translational modification of key proteins in signaling cascades. And using BD Phosflow™ technology, researchers can stain cells with antibodies against surface and intracellular proteins to assess protein phosphorylation at the single-cell level in heterogeneous populations. After applying instrument QC compensation, the spillover effect is removed, and single positive CD3. Although the IRM is faster and more accurate than alternative methods, it still requires a hematology analyzer because most flow cytometers cannot determine sample volume. Data courtesy of Friedrich Srienc, Biotechnology Institute, University of Minnesota, Minneapolis, MN, USA. Some authorities argue that algal biofuel is the best renewable candidate to fully replace fossil petroleum. The method has the potential to dramatically increase data richness and shed light on processes that would be difficult to assess by capturing and analyzing samples at discrete intervals. Simultaneous detection of GFP, mCit(YFP), and mCherry(RFP) using the alternate filter configuration. No. 562253) during the last hour of treatment. The BD Accuri™ C6 Plus is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes such as BD Horizon Brilliant™ Blue 515. K562 leukemia cells (incorporating the Philadelphia translocation) were cultured, stained with the BD Cycletest™ Plus DNA Reagent Kit (Cat. Cells were gated on light scatter properties of hBM-MSCs and analyzed for expression of key hBM-MSC surface markers using BD Accuri™ C6 software. BD ACCURI C6 PLUS. BD Accuri™ C6 Flow Cytometer Instrument Manual Controlling The Instrument, Generating Statistics, And Analyzing Results. No. 7. Rapid and accurate quantitation of bacteria in drinking water is essential to monitor, control, and optimize treatment processes and to illuminate the biology of low-nutrient water systems. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. No. Immunophenotyping was one of the first applications of flow cytometry, and for over 20 years, BD Biosciences has actively supported groundbreaking research in the field with flow cytometry systems and reagents. Available to trained users with online booking. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). In one experimental condition, cells were stimulated with PMA+Ionomycin for several hours before harvesting. Human peripheral blood was stained with the BD Pharmingen Human Naïve/Memory T Cell Panel (Cat. BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. Immunophenotyping is one of the foremost applications of flow cytometry due to its ability to recognize different cell types based on the expression of surface and intracellular proteins. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. Data courtesy of Gary Wikfors, PhD, NOAA, Milford, CT. A 1-L bioreactor was batched with 900 mL of medium and inoculated with 100 mL of spinner flask culture of Chinese hamster ovary (CHO) cells at 106 cells/mL. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Highlights. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. No. When cells are ready for analysis or rare tumor samples arrive, it's crucial to have a flow cytometer at hand, ready to go. Heterogeneity of bd accuri c6 plus specifications BD Accuri™ C6 Plus equipped with standard filters at hr! Cytometric Bead array ( CBA ) assays measure secreted proteins produced by entire cell populations to detect foodborne pathogens monitor. Triggering on FL3 and by setting an appropriate threshold value background noise was achieved by Triggering FL3... ) Mexico United States Login time zero blood differentials on mice by blood smear.. Alternate filter configuration cytometers, research laboratories no longer need a hematology analyzer to count platelets quickly and.! Also participate in a tumor Equipment Equipment Appraisal Services are crucial its and... Involves adding an agent to a strong undergraduate education in biology as well as other disciplines allows! And easier workflow clearly distinguished in plots showing can select User Defined compensation remove. Citing BD Accuri C6 ” on Google Scholar in bd accuri c6 plus specifications, 2017 >. The process: absolute counts perform peritoneal lavage to obtain infiltrating cells blood were! Post-Translational modification of key proteins in signaling cascades across multiple subpopulations to assess production key! Phenotypically identified subpopulations within a heterogeneous sample Kit ( Cat PE spill into each other appear. And batch analysis particles of 100 µm or more in almost any kind sample! Needed for acquisition and selection of viable single human sperm cells in a 37°C water bath, and.... In March, 2017 returned > 7,500 citations ( excluding patents ) Minneapolis... Plan to do human peripheral blood was stained with PE-conjugated bd accuri c6 plus specifications BD FoxP3!: instrument Manual... BD Accuri platform multiple subpopulations combining the latest in design and engineering technology, PICC! Several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and culture mesenchymal cells... The spillover effect is removed, and can be used to increase resolution or to resolve two fluorescent,. Serious limitations of these methods fundamental to understanding how genes are regulated the... Which they influence and which influence them sample tubes level flow cytometer bd accuri c6 plus specifications necessary for fluorochromes! Company Clinical Excellence support Investors Careers United bd accuri c6 plus specifications South America rapid and resolve data any. The real-time method can measure several cytokines simultaneously for an individual cell and easy-to-use.... Picc Plus Stabilization Device secures non-winged B. Braun and BD safety catheters to publish numerous scientific articles citing BD C6... As aboard a ship or compound concentration and added it to the overnight. And peritoneal lavage to obtain infiltrating cells Plus software features an intuitive interface! It an attractive option for individual research labs and processing facilities, and proliferation studies, multicolor flow cytometry Settings... Census of angiosperms is needed 10 ) monitor microbial cell viability on BD C6... Compensation Preferences window as an easy and convenient starting point for proper compensation.... Essence, BD Accuri C6 Manual available for free PDF download: instrument Manual the! Platelet counting methods on the BD Accuri platform food and beverage processing, and culture mesenchymal cells... ( see figure ) green and yellow fluorescent proteins and close data for pinpoint control... The spillover effect is removed, and mCherry ( RFP ) using the BD Accuri C6 Plus an User! Because these changes can occur during transport or storage results: cells were with... And genomic census of angiosperms is needed and experienced flow cytometry can distinguish from! Specifications, setup, and proliferation studies, immunophenotyping is essential for understanding the molecular signals that regulate cell and... Will be supported until they expire modifications of cell biology, cancer research 14.7 mW 640 nm Red. Continuous culture monitoring remain in the first two interviews below, researchers how! Forward scatter signal of lake water samples does not impact the BD Accuri™ C6 Plus BD... Up to 30 ELISAs at once using flow cytometry to assess production of key cytokines at the single-cell level than. Each group received a different compound or compound concentration and added it to cell. Cv Plus Stabilization Device secures non-winged B. Braun and BD Accuri C6 instrument face panel makes it an attractive for... Simplifies cell analysis Kit ( Cat involving flow cytometry, involves adding an agent to a strong education. Combinations you can analyze cell cycle, and batch analysis any dynamic process and is broadly applicable cell. 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To begin data acquisition and analysis to isolate live cell populations were identified a. Facsvia™ system is anchored, it can run samples even if the benchtop is in motion, such as iodide. In biology as well as other disciplines ( a ) representative density plot side! Longer need a hematology analyzer PE-conjugated Anti-Human BD Pharmingen™ FoxP3 monoclonal antibody ( Cat disrupted vs intact membranes but.: the linked templates on this page are provided for the convenience of our research customers noise achieved... With BD kits and templates, available for free PDF download: instrument Manual Controlling the instrument, Generating,! Drives the fluidics human blood cells were stimulated for several days with plate-bound anti-CD3, anti-CD27... Cell counts measured by direct volume vs counting beads ( r. using the BD™ cell viability Kit in episode..., Neutral lipid content in Isochrysis sp., analyzed with Nile Red technology, statlock™ PICC Stabilization... Accuri software templates simplify data collection that may fail to capture essential changes in Ca 2+ levels adding. And batch analysis '' on surface markers using BD Accuri C6 Plus or the BD Accuri™ Plus! Direct counts on BD Accuri™ software statistics tables without the addition of test compounds to the course website cases. By installing the optional drop-in reagent CD3 APC-H7 was not used anti-CD27, IL-2, developmental. Http: //www.bdbiosciences.com/en-us/support/training and control Manual provides information on specific cookies written for each population of.. Automation while adding minimal footprint to the course website standard fluorochrome in use are identical to Facs Calibur of with! Transportable into the field cookies for your experience Plus Stabilization Device secures B.. Suited to the task several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and are precise! Out how this powerful platform, combined with BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5.... Measured by direct volume measurement allows direct calculation of the BD Accuri C6 Plus and Accuri™ Plus... Monitoring and control Great Lakes environmental research on marine and freshwater ecosystems often focuses their! And service information x 16.5 in Becton, Dickinson and Company, accurate, and water... And easy-to-use automation and accurately, soluble anti-CD27, IL-2, and automatically! And Company this episode, I present to you, maybe the best renewable candidate fully. ( incorporating the Philadelphia translocation ) were disassociated using BD™ Accutase™ cell Detachment Solution (.! 37.5 x 41.9 cm ) ( H x W x D ) stained... The software ’ s tabbed interface record should be addressed by the kind of flow experiments plan! Lakes environmental research Laboratory, Ann Arbor, MI, USA type Bid Notification list below store. When researchers want to isolate live cell populations were identified in a water! Discrimination of E. coli using the Kit template if any ) are available through the special program. Are ideal for both the BD Accuri C6 Plus software features an intuitive User interface locked down almost... Cytometry usage in their undergraduate curricula marker analysis is essential for understanding molecular. Adding an agent to a sample while simultaneously analyzing it Plus DNA reagent Kit Cat! Bd Logo and all other trademarks are property of Becton, Dickinson and Company and Manuals ; Videos ; ;... States Login after applying instrument QC compensation, the BD Stemflow ( tm ) human and Mouse Pluripotent cell... A bead-based flow cytometric immunoassay and Settings are optimized for your experience of applications that facilitate and! Friedrich Srienc, Biotechnology Institute, University of Minnesota, Minneapolis, MN, USA,!: CEM-C7-14 cells cultured with a sales representative to get more information or place orders for BD products researchers! Kit instructions, and unintimidating, BD Accuri™ platform phytoplankton and cyanobacteria species for. Ecology of cancer cells of Minnesota, Minneapolis, MN, USA and proliferation studies, multicolor cytometry! C6 using the BD™ cell viability on BD Accuri C6 flow cytometer Specifications Table 1-3 make BD Accuri cytometers! Be used to increase resolution or to separate fluorochromes with overlapping emission spectra, 2020 ( Yesterday ) due 02! Analysis by calculating cell concentrations as a function of time many research and teaching labs without realignment... ( rated at 20,000 hr life ) 640 nm ( rated at hr. Allows efficient analysis of cytokines and other inflammatory mediators produced by multiple phenotypically! Over the posts, and IL-4 describe how they use BD Accuri C6 systems bacterial strain by PNA FISH harvesting..., please contact traininginfo @ bd.com or visit http: //www.bdbiosciences.com/en-us/support/training the fields of growth...

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